Astrovirus ribosomal frameshifting in an infection-transfection transient expression system
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چکیده
منابع مشابه
Astrovirus ribosomal frameshifting in an infection-transfection transient expression system.
Different regions of the human astrovirus frameshift signal were cloned into the rhesus rotavirus VP4 gene and evaluated in an infection-transfection transient expression cell culture system. BHK-21 cells, infected with a vaccinia virus that expresses T7 RNA polymerase (vTF7-3), were transfected with the various astrovirus-VP4 constructs. All constructs were driven by a T7 promoter and containe...
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Many retroviruses use -1 ribosomal frameshifting as part of the mechanism in translational control of viral protein synthesis. Quantitative prediction of the efficiency of -1 frameshifting is crucial for understanding the viral gene expression. Here we investigate the free energy landscape for a minimal -1 programmed ribosomal frameshifting machinery, including the codon-anticodon base pairs at...
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Accurate maintenance of the translational reading frame by ribosomes is essential for the production of functional proteins and unsurprisingly, errors in frame maintenance have been estimated to occur at rates probably lower than 5 x 10 5 per codon (Kurland, 1992). However, an increasing number of examples have been documented where a purposeful shift in reading frame is programmed into the mRN...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1996
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.70.5.2869-2875.1996